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使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Please note that all translations are automatically generated. Click here for the English version.

使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化

DOI:

10.3791/60813-v

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08:02 min

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March 12, 2020

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Damian Jacenik, Marta Zielińska, Sylwia Michlewska, Jakub Fichna, Wanda M. Krajewska

¹Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, ²Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, ³Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Capitoli

00:05Introduction
00:49Colon Sample Preparation
03:12Immunohistochemistry
06:07Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
07:20Conclusion

Summary

Traduzione automatica

该协议提出了完整的经验证的克罗恩病TNBS诱导的鼠模型，以及利用嵌入在石蜡中的正式固定结肠部分的免疫荧光，通过免疫组织化学对雌激素受体进行可视化的方法。

Tags

Estrogen Receptor Immunofluorescence TNBS-induced Crohn’s Disease Mouse Colon Paraffin Embedding Microtome Sectioning Deparaffinization

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