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1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias,Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación,Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture,Universidade de Santiago de Compostela
Capitoli
- 00:00Introduction
- 01:06Synthesis of Magnetic Nanoparticles Conjugated with Feroxamine
- 09:57Bacterial Assay with Y. ENTEROCOLITICA Strains to Quantify the Isolation and Capture of Pathogenic Bacteria with Nanoparticles
- 13:52Risultati
This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.
