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Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
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Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

DOI:
10.3791/60847-v

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05:27 min

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August 13, 2020

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, , , , ,
1Department of Thoracic Surgery,The First Hospital of Jilin University, 2The Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences,University of Colorado Anschutz Medical Campus, 3Key Laboratory of Zoonoses Research, Ministry of Education,Jilin University, 4Department of Cardiology,China-Japan Union Hospital of Jilin University, 5Department of Gastrointestinal, Colorectal and Anal Surgery,China-Japan Union Hospital of Jilin University

Capitoli

  • 00:04Introduction
  • 00:32Vascular Catheterization
  • 03:00Perfusion System Setup
  • 04:11Results: Representative Pre- and Post-Operative Imaging
  • 04:52Conclusion

Summary

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A protocol is described for in situ perfusion of the mouse lower body, including the bladder, the prostate, sex organs, bone, muscle and foot skin.

Tags

In SituClosed Circuit PerfusionLower Abdominal OrgansHind LimbsMiceDrug DeliveryImmune InteractionsCatheterizationAbdominal AortaInferior Vena CavaIliac Arteries And VeinsPerfusion SystemWater-jacketed Moist Chamber

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