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Biochemistry

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JoVE Journal Biochemistry
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
 

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Article DOI: 10.3791/60851-v • 07:10 min • March 13th, 2020
March 13th, 2020 •
1,2,3, 1,4, 5,6, 1
1Research and Innovation, Murdoch University, 2Centre for Digital Agriculture, Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre, Murdoch University

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A method for the untargeted analysis of wheat grain metabolites and lipids is presented. The protocol includes an acetonitrile metabolite extraction method and reversed phase liquid chromatography-mass spectrometry methodology, with acquisition in positive and negative electrospray ionization modes.

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Liquid Chromatography-Mass Spectrometry Metabolomics Analysis Wheat Grain Genome Environment Management Practices Prediction Product Yield Product Quality Third Mobile Phase Metabolite Separation Metabolite Detection Biochemistry Understanding Organism Response To Stress Biomarkers Association Isopropyl Alcohol Viscous Solvent Equilibration Time Composition Increase Chromatographic System Pressure Prevention Analytical Column Protection Grain Preparation Laboratory Blender Grinding Grain Coarsely-ground Grain Removal
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