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Förbättrad livskraft för Ex vivo 3D Hydrogel Kulturer av patient-derived Xenografts i en Perfused mikrofluidisk plattform
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1Department of BioSciences,Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine,The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering,Rice University, 6Department of Diagnostic and Biomedical Sciences,The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research,The University of Texas MD Anderson Cancer Center
Capitoli
- 00:04Introduction
- 01:09PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component
- 03:17Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells
- 06:03Hydrogel Preparation and Microfluidic Plate Seeding
- 10:12Results: Cell Staining and Viability Assessment
- 11:16Conclusion
Detta protokoll demonstrerar metoder för att möjliggöra utökad in vitro-kultur av patient-härledda xenografts (PDX). Ett steg förstärker den totala livskraften hos flercelliga klusterkulturer i 3D-hydrogeler, genom okomplicerat avlägsnande av icke-livskraftiga enstaka celler. Ett sekundärt steg visar bästa praxis för PDX-kultur i en perfused mikrofluidisk plattform.
