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Opsono-Adherence Assay om functionele antilichamen te evalueren in vaccinontwikkeling tegen Bacillus anthracis en andere ingekapselde pathogenen
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
DOI:

13:47 min

May 19, 2020

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Capitoli

  • 00:04Introduction
  • 01:21Plating of the Cell Line, RAW 264.7 and Bacterial Culture
  • 06:29FITC-labeling of Bacteria and Bacterial Opsonization
  • 08:46Adherence Assay and Fluorescent Labeling of Mammalian Cells
  • 10:22Microscopy
  • 11:33Results: Representative Micrographs Collected During an OAA Experiment to Evaluate the Efficacy of an Anthrax Vaccine
  • 13:01Conclusion

Summary

Traduzione automatica

De opsono-therapietrouwtest is een alternatieve methode voor de opsono-fagocytische moordtest om de opsonische functies van antilichamen in de ontwikkeling van vaccins te evalueren.

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