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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
JoVE Journal
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JoVE Journal Genetica
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

DOI:
10.3791/60906-v

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05:45 min

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March 11, 2020

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, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Capitoli

  • 00:04Introduction
  • 00:59Nanopore Data Consensus Sequence Generation
  • 02:33Nanopore Sequencing Error Profile Compensation
  • 03:54Results: Representative Nanopore Sequencing Error Rate Overview
  • 04:58Conclusion

Summary

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We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

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Artificial IntelligenceAI Writing AssistantsCopywritingContent GenerationText-based ContentContent CreationAI-assisted WritingWriter’s BlockContent EfficiencyReal-time SequencingVirus Genetic SequencingNanopore SequencingUsutu VirusError RateRead CoverageSequence DataConsensus SequenceReference-based AlignmentMinimap2SamtoolsKMA

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