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细菌群的延时成像与集体压力反应
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Biologia
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JoVE Journal Biologia
Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
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细菌群的延时成像与集体压力反应

DOI:
10.3791/60915-v

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06:26 min

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May 23, 2020

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, ,
1Department of Molecular Biology & Biochemistry,University of California, Irvine, 2Department of Physics & Astronomy,University of California, Irvine, 3Department of Veterinary and Animal Sciences,University of Copenhagen

Capitoli

  • 00:04Introduction
  • 00:36Swarming Agar Plate Preparation
  • 01:32P. aeruginosa Growth and Plating
  • 03:30Time-Lapse Image Acquisition and Swarm Repulsion Measurement
  • 04:55Results: Representative Time-lapse Swarming and Collective Stress Response Imaging
  • 05:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们详细介绍了一个简单的方法，以产生高分辨率延时电影的伪单一aeruginosa群，响应噬菌体（噬菌体）和抗生素压力使用平板文档扫描仪。这个过程是一种快速而简单的监测蜂群动力学的方法，可应用于研究其他细菌物种的动能和生长。

Tags

Time-lapse ImagingBacterial SwarmingCollective Stress ResponsePseudomonas AeruginosaBacteriophageAntibioticsSwarming Agar PlatesDehumidifierDocument ScannerSwarming DynamicsPhage InfectionAntibiotic TreatmentConcentric Circle Plating

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