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斑马鱼内胚细胞中基于共体显微镜的激光消融和再生测定
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
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斑马鱼内胚细胞中基于共体显微镜的激光消融和再生测定

DOI:
10.3791/60966-v

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11:17 min

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May 20, 2020

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, ,
1Department of Biology,Pace University

Capitoli

  • 00:04Introduction
  • 00:42Larva Mounting
  • 02:16Prospective Target Location
  • 04:02Pre-Ablation Imaging
  • 05:49Laser Ablation
  • 07:03Post-Ablation Imaging and Regeneration Time-Lapse Microscopy
  • 08:25Results: Representative Interneuromast Cell (INMC) Ablation Imaging and Gap Measurement
  • 10:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

激光消融是研究生物系统再生的一项广泛应用技术。提出的方案描述了使用标准激光扫描共生显微镜进行激光消融，以及斑马鱼横向线中再生内泌细胞的后续延时成像。

Tags

Confocal MicroscopeLaser AblationRegenerationZebrafishInterneuromast CellsHair Cell ProgenitorSensory ProgenitorsHearing LossE3 Tricaine SolutionAgaro-solutionGFPLateral LineBright FieldDICEpifluorescent

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