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High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

DOI:
10.3791/60976-v

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08:34 min

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March 12, 2020

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, , , , , , ,
1Stanford Cardiovascular Institute,Stanford University, 2Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

Capitoli

  • 00:05Introduction
  • 00:48Experimental Set-up
  • 02:18Handling the Fish
  • 03:13Image Acquisition and Fish Recovery
  • 05:43Image Analysis
  • 06:31Results: B-Mode Images and Pulse Wave Doppler Images
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a protocol to assess heart morphology and function in adult zebrafish using high-frequency echocardiography. The method allows visualization of the heart and subsequent quantification of functional parameters, such as heart rate (HR), cardiac output (CO), fractional area change (FAC), ejection fraction (EF), and blood inflow and outflow velocities.

Tags

ZebrafishEchocardiographyCardiac FunctionNoninvasiveHigh-resolutionAdult Heart DiseaseDrug ScreensImagingTransducerAnesthesiaMicromanipulator

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