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Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay
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Biologia
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JoVE Journal Biologia
Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

DOI:
10.3791/60983-v

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05:20 min

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June 11, 2020

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, ,
1Department of Biology,University of Maryland Baltimore County

Capitoli

  • 00:04Introduction
  • 00:36Injection of Flies
  • 02:03Dorsal Vessel Dissection
  • 03:07Fixation, Mounting, and Processing
  • 03:50Results: Imaging and Quantification of Phagocytic Events
  • 04:36Conclusion

Summary

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This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.

Tags

Keywords: Phagocytic AbilityHemocytesDrosophila MelanogasterIn Vivo Phagocytosis AssayPhagocytic EventsDissectionGlass NeedlesInjectionFluoro ParticlesPH-sensitive ParticlesStereomicroscopeInsect PinsDissection MediaCuticle ScissorsForcepsDorsal Vessel

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