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脑切片和原细胞培养中金字塔神经元的弹道标记

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

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脑切片和原细胞培养中金字塔神经元的弹道标记

DOI:

10.3791/60989-v

•

09:40 min

•

April 02, 2020

•

Hailong Li, Kristen A. McLaurin, Charles F. Mactutus, Rosemarie M. Booze

¹Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capitoli

00:04Introduction
00:38Preparation of Dil/Tungsten Bead Tubing
02:55Preparation of Brain Sections
04:20Ballistic Labeling and Visualization of Brain Sections
06:16Cell Culture Method
07:12Results: Analysis of Pyramidal Neurons Using Ballistic Labeling Technology
08:44Conclusion

Summary

Traduzione automatica

我们提出了一种用于标记和分析金字塔神经元的协议，这对于评估神经元和树突脊柱的潜在形态变化至关重要，这些变化可能是神经化学和行为异常的基础。

Tags

Keywords: Ballistic Labeling Pyramidal Neurons Brain Slices Primary Cell Culture PVP Solution Tungsten Microcarrier Beads Lipophilic DiIC18(3) Dye Methylene Chloride Sonication Nitrogen Gas Drying Rat Brain Perfusion PBS PFA

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