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Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
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JoVE Journal Neuroscienze
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

DOI:
10.3791/60993-v

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10:12 min

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May 05, 2020

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, , , ,
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv University, 2Sagol School of Neuroscience,Tel Aviv University

Capitoli

  • 00:04Introduction
  • 01:08Microfluidic Chamber Preparation
  • 05:53Neuronal Culture Plating
  • 07:24Axonal Transport
  • 08:19Results: Axonal Transport of Mitochondria and Acidic Compartments in Motor Neurons
  • 09:34Conclusion

Summary

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Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

Tags

Keywords: Axonal TransportMotor NeuronMicrofluidic ChambersNeurodegenerative DiseaseALSAxonal GrowthAxonal DegenerationPDMSChlorotrimethylsilaneVacuum Desiccator

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