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血管化、扩展发育和改进显微镜成像的肾器官和器官培养的优化
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging
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血管化、扩展发育和改进显微镜成像的肾器官和器官培养的优化

DOI:
10.3791/60995-v

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12:49 min

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March 28, 2020

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, , , , ,
1Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine,University of Oulu, 2InfoTech Oulu,University of Oulu, 3Biobank Borealis of Northern Finland,Oulu University Hospital

Capitoli

  • 00:00Introduction
  • 02:00Protocol
  • 08:11Risultati
  • 11:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文描述了研究器官发育的两种方法，一种是改善鸟类胚胎中胆囊膜（CAM）的异种移植设置，允许培养胚胎器官和器官的血管化，以及一种新颖的固定z方向器官培养方法具有改进的实验条件，允许高分辨率延时共聚焦成像。

Tags

Renal OrganoidOrganotypic CultureVascularizationExtended DevelopmentMicroscopy ImagingKidney Organ CultureStem CellsKidney DevelopmentBlood FiltrationChorioallantoic MembraneConfocal ImagingOrgan Culture MethodEmbryonic KidneysRenal Organoids

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