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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
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Capitoli
- 00:04Introduction
- 00:50Extraction of Potential Plant Phytosteroids
- 02:29Human Estrogen Receptor β Transfection Assay
- 04:25Results: ERβ Reporter Assay System for Phytoestrogen Activity Screening of Fruits and Vegetables
- 05:19Conclusion
We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.
