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1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital,University of Sydney, 2HTRG – Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery,Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine,Michigan State University
Capitoli
- 00:04Introduction
- 00:39Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
- 04:18Tissue Processing: Spleen
- 05:16Tissue Processing: Lymph Node and Bone Marrow
- 06:42Tissue Processing: Synovial Tissue
- 07:57Monocyte and T cell Subset Panel Immunostaining
- 10:28Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
- 11:45Conclusion
Hier beschreiben wir ein detailliertes und reproduzierbares Flusszytometrieprotokoll zur Identifizierung von Monozyten-Makrophagen- und T-Zell-Subsets mit extra- und intrazellulären Färbetests innerhalb der murinen Milz, des Knochenmarks, der Lymphknoten und des Synovialgewebes unter Verwendung eines etablierten chirurgischen Modells der murinen Arthrose.
