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1Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 2University of California Berkeley-UCSF Graduate Program in Bioengineering,University of California San Francisco, 3Department of Pharmaceutical Chemistry,University of California San Francisco, 4California Institute for Quantitative Biosciences,University of California San Francisco, 5Chan Zuckerberg Biohub
Capitoli
- 00:04Introduction
- 00:29Yeast Encapsulation in Hydrogels Using Device A
- 01:39Breaking and Washing Hydrogels
- 02:58Yeast Colony Lysis
- 04:04mRNA Capture Using Device B
- 05:27Results: High-Throughput Screening of C. albicans
- 07:24Conclusion
En flaskehals i den "design-build-test" cyklus af mikrobiel teknik er den hastighed, hvormed vi kan udføre funktionelle skærme af stammer. Vi beskriver en høj-throughput metode til stamme screening anvendes til hundreder til tusindvis af gær celler pr eksperiment, der udnytter dråbe-baserede RNA sekventering.
