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使用阿基-树脂辅助捕获检测蛋白质S-囊肿
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Biochimica
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JoVE Journal Biochimica
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
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使用阿基-树脂辅助捕获检测蛋白质S-囊肿

DOI:
10.3791/61016-v

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08:31 min

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April 10, 2020

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, , ,
1Department of Biochemistry and Molecular Biology,McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School

Capitoli

  • 00:04Introduction
  • 01:25Cell Lysate Preparation
  • 02:26Acyl-Resin Assisted Capture (Acyl-RAC): Free Thiol Group Blocking
  • 03:54Acyl-RAC: Hydroxylamine (HAM) Cleavage and S-Acylated Protein Capture
  • 05:52S-Acylated Protein Elution and Detection
  • 06:52Results: Representative S-Acylated Protein Detection
  • 07:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

Acyl-RAC（阿西尔-树脂辅助捕获）是一种高度敏感、可靠且易于执行的方法，用于检测各种生物样品中半胱氨酸残留物（S-acycyy）的可逆脂改性。

Tags

Keywords: Acyl-resin Assisted CaptureAcyl-RACProtein S-acylationThioester Bond CleavageChloroform-methanol PrecipitationProtein PelletCell LysisProtein Concentration EstimationBradford AssayBCA Assay

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