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使用流细胞测定量化人类诺如病毒样颗粒与准细菌结合
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
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使用流细胞测定量化人类诺如病毒样颗粒与准细菌结合

DOI:
10.3791/61048-v

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07:02 min

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April 29, 2020

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,
1Microbiology and Cell Science Department,University of Florida

Capitoli

  • 00:04Introduction
  • 00:37Correlating Optical Density and Bacterial Concentration
  • 02:14VLP Attachment
  • 03:29Antibody Staining
  • 05:19Results: Quantifying VLP Binding
  • 06:15Conclusion

Summary

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该协议的目标是量化真核病原体人类诺如病毒与细菌的结合。在进行初始病毒-细菌附件测定后，流细胞测定用于检测种群内的病毒结合细菌。

Tags

Virus-bacterial InteractionsHuman NorovirusVirus-like ParticlesFlow CytometryEnterobacter CloacaeBacterial QuantificationSerial DilutionsOptical DensityColony Forming Units

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