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Biochemistry

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JoVE Journal Biochemistry
A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
 

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

Article DOI: 10.3791/61053-v • 11:32 min • May 4th, 2020
May 4th, 2020 •
1, 1
1Department of Chemistry, McGill University

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Lanthipeptide synthetases catalyze multistep reactions during the biosynthesis of peptide natural products. Here, we describe a continuous, bottom-up, hydrogen-deuterium exchange mass spectrometry (HDX-MS) workflow that can be employed to study the conformational dynamics of lanthipeptide synthetases, as well as other similar enzymes involved in peptide natural product biosynthesis.

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Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Peptide Biosynthetic Enzymes Protein Conformational Dynamics Protein Labeling Nanomolar Amount Undeuterated Reference Samples Quench Buffer Liquid Chromatography Mass Spectrometry Proteomic Software Program Amino Acid Sequence FASTA Format Data Acquisition Type Lock Mass For Charge 2 Field
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