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Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
 

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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We present a detailed protocol for potato virus X (PVX)-based microRNA silencing (VbMS) system to functionally characterize endogenous microRNAs (miRNAs) in potato. Target mimic (TM) molecules of miRNA of interest are integrated into the PVX vector and transiently expressed in potato to silence the target miRNA or miRNA family.

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Keywords: Potato Virus X MicroRNA Silencing VbMS Virus-based MicroRNA Silencing MiRNA Functional Characterization Short Tandem Target Mimic PVX Ligation Independent Cloning
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