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Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
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Biologia
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JoVE Journal Biologia
Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

DOI:
10.3791/61073-v

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09:17 min

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October 10, 2020

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, , , ,
1Department of Pathology and Laboratory Medicine, College of Medicine,University of Cincinnati

Capitoli

  • 00:00Introduction
  • 01:14Preparation & Materials
  • 02:49Heart Extraction
  • 04:15Heart Digestion & Single-Cell Suspension
  • 05:59Cardiomyocyte Plating & Transfection
  • 08:13Risultati
  • 08:50Conclusion

Summary

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Here, we present a protocol for the isolation, transfection, and long-term culture of adult mouse and rat cardiomyocytes.

Tags

CardiomyocytesAdult Mouse And RatIsolationTransfectionLong-term CultureProliferative PotentialGene ExpressionCytokine ExpressionInjury ResponseOptimized MethodSurvival RateTransfection EfficiencySurgical InstrumentsProfusion ApparatusHeparinMyocyte BufferEnzyme SolutionAnesthesia

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