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A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
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Capitoli
- 00:04Introduction
- 00:45PDMS Fabrication
- 02:15Chip Assembly and Sterilization
- 03:47Mechanical Loading Device Assembly
- 04:33Loading Experiment
- 06:23Results: Functional Activity and Effects of Mechanically Induced Strain
- 07:48Conclusion
Here, we present protocols for analyzing bone remodeling within a lab-on-a-chip platform. A 3D printed mechanical loading device can be paired with the platform to induce osteocyte mechanostransduction by deforming the cellular matrix. The platform can also be used to quantify bone remodeling functional outcomes from osteoclasts and osteoblasts (resorption/formation).
