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Cancer Research

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JoVE Journal Cancer Research
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
 

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Article DOI: 10.3791/61077-v • 06:52 min • July 22nd, 2020
July 22nd, 2020 •
1, 4, 1, 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

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Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.

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Driver Genes Colorectal Cancer Tumorspheres RNA-Seq Bioinformatical Tools Overexpressed Genes Molecular Mechanism Cancer Stemness Inverted Microscope Digital Cell Imaging System Diameter Measurement Trypsin Treatment Cell Count Hemocytometer Centrifugation Antibody Incubation Flow Cytometry Analysis RNA Isolation
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