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A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
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Biologia
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JoVE Journal Biologia
A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

DOI:
10.3791/61081-v

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07:10 min

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June 12, 2020

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, ,
1Department of Biology,The University of Akron, 2Department of Geosciences,The University of Akron

Capitoli

  • 00:04Introduction
  • 01:10Tissue Sectioning
  • 03:38Sample Coring
  • 05:29Results: SRμCT Reconstructed Slices of the Anterior Aspect of the Left Femur from Two Individuals
  • 06:37Conclusion

Summary

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We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

Tags

Keywords: Bone SpecimenCortical BoneSynchrotron Micro-CTSample ProcurementSectioningCoringImage ProcessingHigh-throughputThermal Epoxy ResinMicroscope SlideSlow Speed Sectioning SawCross-sectionRock CoringFossil CoringHard Material CoringEarth’s CrustUpper MantleRheology

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