Rivista
/
Biologia
/
Live-Cell Imaging af livscyklus bakteriel Predator Bdellovibrio bacteriovorus ved hjælp af Time-Lapse Fluorescens mikroskopi
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Live-Cell Imaging af livscyklus bakteriel Predator Bdellovibrio bacteriovorus ved hjælp af Time-Lapse Fluorescens mikroskopi

DOI:
10.3791/61105-v

•

08:56 min

•

May 08, 2020

•

, ,
1Department of Molecular Microbiology, Faculty of Biotechnology,University of Wroclaw

Capitoli

  • 00:04Introduction
  • 00:34B. bacteriovorus Lysate Preparation
  • 01:47Host Cell Preparation
  • 02:32Time-Lapse Fluorescence Microscopy Layout Preparation
  • 03:36Time-Lapse Fluorescence Microscopy
  • 05:30Time-Lapse Image Processing and Movie Generation
  • 07:11Results: Representative B. bacteriovorus Life Cycle Captured by Time-Lapse Fluorescence
  • 08:16Conclusion

Summary

Traduzione automatica

Traduzione automatica

Præsenteret her er en protokol, der beskriver overvågning af hele livscyklus aggressiv bakterie Bdellovibrio bakterien ved hjælp af time-lapse fluorescens mikroskopi i kombination med en agarose pad og celle-imaging retter.

Tags

Live-cell ImagingBdellovibrio BacteriovorusTime-lapse Fluorescence MicroscopyBacterial PredatorLife CyclePrey CellsAgarose PadCalcium HEPES BufferOptical DensityCentrifugationFluorescence MicroscopyPrey LysisMulti-attack Phase

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/61105?article_type=v

Monitoring Actin Disassembly with Time-lapse Microscopy
check_url/it/61105?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/it/61105?article_type=v

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
check_url/it/61105?article_type=v

Kvantitative levende celler fluorescens-mikroskopi Analyse af Fission Yeast
check_url/it/61105?article_type=v

Kvantitativ analyse af Random migration af celler Brug af Time-lapse Video Microscopy
check_url/it/61105?article_type=v

Erhvervelse Fluorescens Time-lapse film fra Spirende Gær og analysere encellede Dynamics hjælp poder
check_url/it/61105?article_type=v

Visualisering af kraniofaciale udvikling i sox10: Kaede Transgene zebrafisk linje ved hjælp af Time-lapse konfokal mikroskopi
check_url/it/61105?article_type=v

In Vivo 4-Dimensional sporing af hæmatopoietisk stamcelletransplantation og stamcelle i Voksen Mouse calvarie knoglemarv
check_url/it/61105?article_type=v

Funktionel billeddannelse af Viral transskription fabrikker ved hjælp af 3D Fluorescens mikroskopi
check_url/it/61105?article_type=v

En alsidig metode til montering Arabidopsis blade til Intravital Time-lapse Imaging

Read Article