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Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
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JoVE Journal Bioingegneria
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

DOI:
10.3791/61106-v

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08:13 min

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February 27, 2021

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, , , ,
1Laboratoire Matière et Systèmes,Complexes MSC, UMR 7057, CNRS & University Paris Diderot, 2Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science,LVTS, INSERM, UMR 1148, 3Services de Biochimie et Médecine Nucléaire,Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

Capitoli

  • 00:05Introduction
  • 00:58Synthesis of Magnetic Nanoparticles
  • 02:37Culture and Magnetic Labeling of Stem Cells
  • 03:33Formation of Stem Cell-Spheroids
  • 04:51Quantification of Magnetic Nanoparticles Using a Vibrating Sample Magnetometer (VSM)
  • 05:46Results: VSM Measures Uptake and Biodegradation of Nanoparticles
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).

Tags

Magnetic NanoparticlesMagnetometryCellular IncorporationBiodegradationIron Oxide NanoparticlesSynthesisBiomedical ApplicationsMagnetismContaminationMagnetometerLiquid SamplesBiological SamplesIron(III) AcetylacetonateMicrowave ReactorNeodymium MagnetHydrochloric AcidCoating MoleculesHuman Mesenchymal Stem CellsRPMI Medium

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