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神经干细胞向大鼠和小鼠大脑的动脉内传递：对脑缺血的应用
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Neuroscienze
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JoVE Journal Neuroscienze
Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia
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神经干细胞向大鼠和小鼠大脑的动脉内传递：对脑缺血的应用

DOI:
10.3791/61119-v

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14:53 min

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June 26, 2020

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, , ,
1College of Public Health,Shaanxi University of Chinese Medicine, 2Spinal Cord and Brain Injury Research Center, Department of Physiology,University of Kentucky

Capitoli

  • 00:00Introduction
  • 00:24Preparation of Injection Cathedters and Surgical Hooks
  • 02:48Culture of GFP (+) Neural Stem Cells (NSCs)
  • 03:52Ischemic Stroke Surgery
  • 09:24NSC Injection
  • 13:07Representative Results
  • 14:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

报告缺血性中风后，通过普通胡萝卜动脉（小鼠）或外体皮动脉（大鼠）提供神经干细胞的方法，可适应注射溶液或悬浮液。注射的细胞广泛分布于整个脑帕伦奇玛，在分娩后可检测到长达30天。

Tags

Intra-arterial DeliveryNeural Stem CellsCerebral IschemiaStrokeCatheter DesignSurgical HooksStem Cell InjectionGFP-positive Neural Stem Cells

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