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Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
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Capitoli
- 00:04Introduction
- 00:41Influenza A Virus Stock Preparation
- 02:38Cell Infection Quantity Determination
- 04:59CIT50 Value and Multiplicity of Infection (MOI) Correlation
- 06:01Influenza A Virus (IAV) Kinetics
- 07:10Results: Representative Influenza Virus External Host Survival Monitoring
- 08:20Conclusion
Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.
