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Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
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JoVE Journal Bioingegneria
Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

DOI:
10.3791/61134-v

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06:56 min

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September 28, 2020

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, , , , ,
1Regenerative Medicine Research,Texas Heart Institute, 2Institute of Biomedical Sciences,Federal University of Rio de Janeiro, 3Biological Systems and Engineering,Lawrence Berkeley National Lab, 4Materials Engineering Division,Lawrence Livermore National Lab

Capitoli

  • 00:00Introduction
  • 01:26Laminin Polymerization Using Low pH Buffers
  • 03:14Laminin Polymerization Using Dystroglycan Expressing Cells
  • 04:00Characterization of Laminin Constructs via Box Counting Dimension
  • 04:48Results: Ln1 Polymerization
  • 06:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe three methods to generate Ln1 polymers with fractal properties that signal to cells differently compared to unpolymerized Ln1.

Tags

Keywords: Laminin-111Extracellular MatrixFractal MicrostructurePolymerized Laminin-1Epithelial Cell DifferentiationCell-matrix InteractionSpinal Cord InjuryHeart FailureBreast CancerPolymerization BufferDystroglycan

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