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דור מדרגי של אורגנואידים המוח הקטן בוגר מתאי גזע פלורופוטנטיים אנושיים ואפיון על ידי אימונוסטיין
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1iBB – Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico,Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina,Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus,Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA
Capitoli
- 00:04Introduction
- 00:23Seeding the Bioreactor
- 02:06Differentiation and Maturation of Aggregates
- 03:26Preparing Organoids for Cryosectioning
- 05:47Immunostaining
- 08:27Results: Efficient Cerebellar Differentiation and Maturation
- 10:09Conclusion
