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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

DOI:
10.3791/61149-v

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07:51 min

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June 03, 2020

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, , ,
1Department of Biology,Tufts University

Capitoli

  • 00:04Introduction
  • 00:35Growing Germ-free Cabbages
  • 03:03Inoculating the Phyllosphere with Microbial Solutions
  • 04:56Preparing Sterile Vegetable Extract
  • 05:59Results: Growth of Microbial Inoculates in the Napa Cabbage Phyllosphere and Sterile Vegetable Extract
  • 07:20Conclusion

Summary

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A method of growing germ-free Napa cabbages has been developed which enables researchers to evaluate how single microbial species or multispecies microbial communities interact on cabbage leaf surfaces. A sterile vegetable extract is also presented which can be used to measure shifts in community composition during vegetable fermentation.

Tags

Gnotobiotic SystemMicrobiome AssemblyPhyllosphereVegetable FermentationSterile Vegetable ExtractPhyllo Sphere CommunitiesSurface SterilizeCabbage SeedsMS BrothSterile Cabbage SeedGerm-free CabbageSterility TestCabbage Homogenate

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