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Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
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JoVE Journal Bioingegneria
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

DOI:
10.3791/61161-v

•

07:06 min

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May 14, 2020

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, , ,
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Capitoli

  • 00:04Introduction
  • 00:47Acoustic Wave Separator (AWS) Preparation
  • 01:48System Priming
  • 03:00AWS Operation
  • 04:09Ending AWS
  • 05:13Results: Representative Turbidity Measurements and Cell Removal Efficiency Comparison
  • 06:37Conclusion

Summary

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Presented here is a protocol for the primary clarification of CHO cell culture using an acoustic separator. This protocol can be used for the primary clarification of shake flask cultures or bioreactor harvests and has the potential application for continuous clarification of the cell bleed material during perfusion bioreactor operations.

Tags

CHOHarvested Cell Culture FluidAcoustic Wave SeparatorPrimary ClarificationTurbidityCell-freeContinuous BioprocessingFiltrationChromatography

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