Rivista
/
Biologia dello sviluppo
/
CCL5诱导小鼠骨膜骨骼干细胞迁移的实时成像
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
Please note that all translations are automatically generated. Click here for the English version.

CCL5诱导小鼠骨膜骨骼干细胞迁移的实时成像

DOI:
10.3791/61162-v

•

06:10 min

•

September 16, 2020

•

, , ,
1Department of Molecular & Human Genetics,Baylor College of Medicine, 2Center for Skeletal Biology,Baylor College of Medicine, 3Department of Pathology & Immunology,Baylor College of Medicine

Capitoli

  • 00:04Introduction
  • 00:39Surgical Procedure
  • 02:11CC Chemokine Ligand 5 (CCL5) Treatment
  • 02:50lntravital Imaging Preparation
  • 03:41Time-Lapse lmaging
  • 04:38Results: Representative Real-Time In Vivo Mx1 Alpha-SMA Periosteal Skeletal Stem Cells (P-SSC) Migration Imaging
  • 05:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了使用活体动物活体显微镜实时检测CCL5介导的骨膜骨骼干细胞迁移。

Tags

Real-time ImagingCCL5Periosteal Skeletal Stem CellsMigrationIn Vivo Cell TrackingMicrofractureChemoattractantIntravital Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

骨骼肌损伤诱导衰老和体内重编程的评价

多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化

活完好斑马鱼的单细胞 Photoconversion

Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

人诱导多能干细胞体外生成体细胞衍生物的研究

评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C） 损伤的反应

在免疫缺陷小鼠中表演人类骨骼肌异种移植物

Read Article