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来自成人大鼠尿膀胱的原发神经元和胶质的隔离与培养

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

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来自成人大鼠尿膀胱的原发神经元和胶质的隔离与培养

DOI:

10.3791/61177-v

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06:18 min

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May 23, 2020

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Rui Wang, Zi-tong Huang, Wen-kang Ren, Jiao Zhang, Yao Zhang, Bo Tan, Ping Huang², Hong-ying Cao²

¹School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine, ²Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine,Guangzhou University of Chinese Medicine, ³School of Basic Medical Sciences,Guangzhou University of Chinese Medicine

Capitoli

00:04Introduction
00:43Bladder Harvest
02:11Two-step Bladder Digestion
04:14Results: Phase-contrast and Immunofluorescence Images of Primary Cells Isolated from the Rat Bladder
05:36Conclusion

Summary

Traduzione automatica

该协议试图建立一个可重复的协议，为初级神经元和胶质隔离从大鼠膀胱进一步细胞实验。

Tags

Keywords: Primary Neurons Adult Rat Urinary Bladder Cell Culture Co-culture Isolation Digestion Krebs Solution Centrifugation

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