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Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

DOI:
10.3791/61178-v

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05:59 min

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May 14, 2020

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, ,
1Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University,Harbin Medical University, 2Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine,University of Calgary

Capitoli

  • 00:04Introduction
  • 00:40Preparation of Materials
  • 01:11Preparation of the Animal for Surgery
  • 01:47Surgical Suturing of Afferent Lymphatic Vessels
  • 03:47Tracking the Lymph Flow
  • 04:31Results: Selective Prevention of Lymph Flow
  • 05:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is presented.

Tags

Keywords: Lymph FlowLymphatic VesselsAfferent Lymphatic VesselsPopliteal Lymph NodesIntradermal InjectionEvans Blue DyeSutureMouseAnesthesiaMicrosurgery

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