Rivista
/
Immunologia e infezioni
/
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

DOI:
10.3791/61180-v

•

09:16 min

•

June 18, 2020

•

, , ,
1Department of Medicine,Monash University, 2Monash Micro Imaging,Monash University, 3Immunology Department,Monash Health

Capitoli

  • 00:04Introduction
  • 01:00Extracellular DNA (ecDNA) Staining
  • 03:50Staining Analysis
  • 07:18Results: Representative ecDNA Identification and Quantification
  • 08:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.

Tags

Supervised Machine LearningSemi-quantificationExtracellular DNAGlomerulonephritisKidney HistologyAntigen RetrievalImmunofluorescence StainingSudan Black Quenching

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/61180?article_type=v

Neutrophil Extracellular Traps: How to Generate and Visualize Them
check_url/it/61180?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/it/61180?article_type=v

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
check_url/it/61180?article_type=v

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
check_url/it/61180?article_type=v

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
check_url/it/61180?article_type=v

Ratiometric Imaging of Extracellular pH in Dental Biofilms
check_url/it/61180?article_type=v

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
check_url/it/61180?article_type=v

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
check_url/it/61180?article_type=v

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
check_url/it/61180?article_type=v

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

Read Article