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Measuring Crop Motility and Food Passaging in Drosophila

JoVE Journal
Biologia

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JoVE Journal Biologia

Measuring Crop Motility and Food Passaging in Drosophila

Measuring Crop Motility and Food Passaging in Drosophila

DOI:

10.3791/61181-v

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06:13 min

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May 09, 2020

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Jiadong Cai*^1,2, Junmeng Xi*^1,2, Youheng Wei²

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China,Yangzhou University, ²College of Bioscience and Biotechnology,Yangzhou University

Capitoli

00:04Introduction
00:48Preparing Experimental Flies
01:39Counting Crop Contractions
02:21Feeding Flies with Dyed Food
02:57Dissecting Flies
03:53Calculating Dye Amounts in Crop and Gut
04:40Results: Crop Contraction and Food Distribution of nprl2 Mutant Flies
05:22Conclusion

Summary

Traduzione automatica

The goal of this protocol is to measure crop contraction and quantify food distribution in the Drosophila gut.

Tags

Crop Motility Food Passaging Drosophila Digestive Function Gastrointestinal Tract Phenotype Carbon Dioxide Anesthesia Crop Contraction Blue Dye Maintenance Food Starvation

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