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Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

DOI:
10.3791/61243-v

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07:22 min

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May 23, 2020

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,
1Division of Biology,Kansas State University

Capitoli

  • 00:04Introduction
  • 01:05Worm Sample Collection
  • 01:59Worm Pellet Extract Preparation
  • 03:14Immunoprecipitation
  • 05:39Results: Representative Caenorhabditis elegans Protein Extract Co-Immunoprecipitation
  • 06:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

This method describes a protocol for high-throughput protein extract preparation from Caenorhabditis elegans samples and subsequent co-immunoprecipitation.

Tags

Keywords: Protein ExtractionCo-immunoprecipitationCaenorhabditis ElegansWorm Sample CollectionM9 BufferLysis BufferBead Mill HomogenizerProtein Extract PreparationImmunoprecipitationEndogenous ProteinsEndogenously Tagged ProteinsOverexpressed ProteinsGenetic Backgrounds

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