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Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
JoVE Journal
Biologia
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JoVE Journal Biologia
Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

DOI:
10.3791/61256-v

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07:09 min

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May 10, 2020

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, , , , , , , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh, 2Research & Development,STEMCELL Technologies Inc

Capitoli

  • 00:05Introduction
  • 00:29Human Pluripotent Stem Cell Seeding
  • 03:34Differentiating hPSCs to Hepatic Progenitors on Laminin-521
  • 04:55Results: Hepatic Progenitor Differentiation from hPSCs
  • 06:26Conclusion

Summary

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The goal of this article is to provide a standardized approach to induce human hepatic progenitor differentiation from pluripotent stem cells. The development of this procedure with ready-to-use media formulations offer the user a facile system to generate human liver cells for biomedical research and translation.

Tags

Hepatic ProgenitorPluripotent Stem CellsDefined Differentiation SystemHepatic DifferentiationLiver BiologyLarge-scale ProductionLaminin-521ROCK InhibitorEnzyme-free DissociationSingle Cell SuspensionViable Cell Count

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