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测量小核苷酸基质的多核苷酸磷酸化的非放射性测定
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Biochimica
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JoVE Journal Biochimica
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
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测量小核苷酸基质的多核苷酸磷酸化的非放射性测定

DOI:
10.3791/61258-v

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06:49 min

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May 08, 2020

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1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Capitoli

  • 00:05Introduction
  • 00:29In Vitro RNA Kinase Reaction
  • 01:27Gel Electrophoresis
  • 04:53Results: Quantification of RNA Phosphorylation
  • 06:06Conclusion

Summary

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该协议描述了一种非放射性检测，用于测量小DNA和RNA基质上多核苷酸激酶（PNKs）的激酶活性。

Tags

Polynucleotide KinasePhosphorylationDNARNASubstrateNonradioactive AssayAcrylamide GelDenaturing GelUreaTBE BufferRNA Enzyme Kinase ReactionsATP SubstrateQuenchingDownstream Analysis

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