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Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
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Biologia
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JoVE Journal Biologia
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

DOI:
10.3791/61259-v

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08:46 min

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August 01, 2020

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, , ,
1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India

Capitoli

  • 00:00Introduction
  • 00:52EMS Mutagenesis (1-3 months)
  • 03:10High Throughput Screening (8 months)
  • 05:21Data Analysis and Results (1-3 months)
  • 06:46Conclusion and Final Thoughts

Summary

Traduzione automatica

Traduzione automatica

A forward genetic screen based on Ca2+ elevation as a read-out leads to identification of genetic components involved in calcium dependent signaling pathways in plants.

Tags

Keywords: Forward Genetic ScreenCalcium SignalingAequorinArabidopsisEMS MutagenesisPhenotype ScreeningGene Mapping

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