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Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus
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Neuroscienze
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JoVE Journal Neuroscienze
Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

DOI:
10.3791/61282-v

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09:36 min

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September 09, 2020

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, ,
1Department of Comparative Biomedical Sciences,Louisiana State University School of Veterinary Medicine

Capitoli

  • 00:05Introduction
  • 01:13Craniotomy and Animal Preparation
  • 02:16AAV Cocktail Injection
  • 03:08In Vivo Neural Recordings with Optogenetics
  • 05:20Amplifier and Filter Settings
  • 07:23Results: VTA Glutamate Projections to the Hippocampus Modulate CA1 Activity
  • 08:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

The current protocol demonstrates a simple method for tracing of ventral tegmental area (VTA) glutamate projections to the hippocampus. Photostimulation of VTA glutamate neurons was combined with CA1 recording to demonstrate how VTA glutamate terminals modulate CA1 putative pyramidal firing rate in vivo.

Tags

Keywords: In Vivo TracingVentral Tegmental AreaGlutamate TerminalsHippocampusOptogeneticsStereotaxic SurgeryExtracellular RecordingAdeno-associated VirusNeurocircuitsNeural Pathways

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