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Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
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1Microbiological Analysis Team, Group for Biometrology,Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy,Chungnam National University (CNU), 3Stem Cell Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection,Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science,University of Science & Technology (UST)
Capitoli
- 00:04Introduction
- 00:34Bacterial Cell Culture and Lactobacillus Cell-Free Supernatant (LCFS) Preparation
- 02:18Colorectal Cancer Cell Line Preparation
- 03:12Spheroid Formation
- 03:493D colorectal Cancer Cell LCFS Treatment
- 04:26Results: Representative Effects of LCFS Co-Culture on Spheroid Formation and Viability
- 05:22Conclusion
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
Tags
Keywords:
Cell DeathCell-free SupernatantProbioticsThree-dimensional SpheroidColorectal CancerLactobacillusAnti-cancer EffectIn Vivo MimicryMRS AgarAnaerobic ChamberMRS BrothL-cysteinePBSShaking IncubatorOD620CentrifugationRPMI 1640Fetal Bovine Serum0.22 Micron StrainerColorectal Cancer Cell LinesMonolayersTrypsin-EDTAGrowth Medium
