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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Biochimica
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JoVE Journal Biochimica
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

DOI:
10.3791/61298-v

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07:31 min

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July 16, 2020

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, , , ,
1Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, 2Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Capitoli

  • 00:05Introduction
  • 00:54Native Cell Membrane Nanoparticle (NCMN) Preparation
  • 03:04Negative Stain Grid Preparation
  • 04:55Results: Representative Negative Stain and Electrophoresis Gel images of Purified Acriflavine Resistance B (AcrB) Constructs
  • 06:52Conclusion

Summary

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Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron microscopy.

Tags

Native Cell Membrane NanoparticlesMembrane Protein-protein Interaction AnalysisElectron MicroscopyMembrane Protein StructureNative EnvironmentHigh-resolution Structure DeterminationMembrane Active PolymerNickel NTA ColumnFast Protein Liquid ChromatographyNegative Stain Grids

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