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1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow, 2Department of Histology,Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow
Capitoli
- 00:04Introduction
- 01:03Isolation of Porcine Cumulus-oocyte Complexes (COCs)
- 02:35Encapsulation in Fibrin-alginate Hydrogel Beads
- 04:48Encapsulation in Super-hydrophobic Fluorinated Ethylene Propylene (FEP) Microbioreactors
- 06:17Results: Morphology and Viability of COCs after Encapsulation
- 07:15Conclusion
Presentert her er to protokoller for innkapsling av svinoocytter i 3D kulturforhold. I den første, cumulus-oocyte komplekser (COCs) er innkapslet i fibrin-alginat perler. I den andre er de omsluttet av fluorerte etylenpropylenpulverpartikler (mikrobioreaktorer). Begge systemene sikrer optimale forhold for å opprettholde sin 3D-organisasjon.
