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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
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Capitoli
- 00:04Introduction
- 00:38Plasmid DNA Linearization
- 01:26Full-Length Hepatitis E Virus (HEV) Genotype 3 p6 DNA In Vitro Transcription and RNA Purification
- 02:35HepG2 Cell Preparation
- 03:47HepG2 Cell Electroporation
- 05:40Intra- and Extracellular Cell Culture Derived HEV (HEVcc) Particle Harvest
- 07:10HepG2/C3A Cell Infection
- 08:47Results: Representative High Titer HEV Stock Preparation and Characterization
- 09:46Conclusion
Described here is an effective method on how to produce high viral titer stocks of hepatitis E virus (HEV) to efficiently infect hepatoma cells. With the presented method, both non-enveloped, as well as enveloped viral particles can be harvested and used for inoculating various cell lines.
