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Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research
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Capitoli
- 00:04Introduction
- 00:35Preparation for PDX-Derived Organoid Culture
- 01:57IC50 Assay
- 03:03Results: Morphology, Transcriptome Expression, and Pharmacological Properties of PDXO-CR2110
- 04:16Conclusion
A method is described to create organoids using patient-derived xenografts (PDX) for in vitro screening, resulting in matched pairs of in vivo/in vitro models. PDX tumors were harvested/processed into small pieces mechanically or enzymatically, followed by the Clevers’ method to grow tumor organoids that were passaged, cryopreserved and characterized against the original PDX.
