Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Xiaoyue Sun; Xiaoqiang Yang; Chui Zeng; Fayed Attia Koutb Megahed; Qi Zhou; Tingdang Liu; Waqas Iqbal; Pingnan Sun; Xiaoling Zhou

doi:10.3791/61414

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Immunologia e infezioni

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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

DOI:

10.3791/61414-v

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09:02 min

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June 05, 2020

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Xiaoyue Sun*^1,2,3, Xiaoqiang Yang*^1,2,3,4, Chui Zeng^2,3, Fayed Attia Koutb Megahed^2,3,5, Qi Zhou^2,3, Tingdang Liu^2,3, Waqas Iqbal^2,3, Pingnan Sun^2,3, Xiaoling Zhou^2,3

¹Stem Cell Research Center,Shantou University Medical College, ²The Center for Reproductive Medicine,Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College, ⁴Medical Research Center, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University, ⁵Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute,General Autority – City of Scientific Researches and Technological Applications

Capitoli

00:04Introduction
01:36HepG2.2.15 Cell Culture
03:04Detection of HBV DNA in Supernatant Concentrate
04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
07:47Conclusion

Summary

Traduzione automatica

This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

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