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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

DOI:
10.3791/61433-v

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09:20 min

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August 11, 2020

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, , , ,
1Department of Mechanical Engineering,University of Kentucky, 2Department of Chemistry,University of Kentucky, 3Department of Physiology,University of Kentucky, 4UK Light Microscopy Core,University of Kentucky

Capitoli

  • 00:04Introduction
  • 00:48Atomic Force Microscopy (AFM)
  • 04:32Confocal Microscopy
  • 06:42Clean Up
  • 07:14Results: Representative Conpokal Live Cell Imaging
  • 08:43Conclusion

Summary

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Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.

Tags

ConpokalConfocal MicroscopyAtomic Force MicroscopyNear Simultaneous Dual-microscopyMechanobiologyAFM CantileverAFM ChipGlass BlockZ Stepper MotorBright-field MicroscopyManual MicrometersLookup TablesAFM Tip

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